Ascorbate Peroxidase (APX) Activity Assay Kit – 100 Tests | Bioquochem
The Ascorbate Peroxidase (APX) Assay Kit (cat. KB03036-100T) enables measurement of ascorbate peroxidase (APX) enzyme activity in plant tissues and cell extracts, a key enzyme in the ascorbate-glutathione cycle (Halliwell-Asada pathway).
Ascorbate peroxidase (APX) catalyzes the reduction of H₂O₂ using ascorbate (vitamin C) as the electron donor, playing a crucial role in plant antioxidant defense against oxidative stress induced by drought, salinity, heat, and pathogen attack. The kit measures the decrease in ascorbate absorbance at 290 nm as APX consumes ascorbate during H₂O₂ reduction. Activity is expressed as nmol ascorbate oxidized/min/mg protein.
Key Features
- Specific assay for APX activity in plant systems
- Suitable for leaves, roots, seedlings, and other plant tissue extracts
- Essential for abiotic stress and plant oxidative stress research
- Pack size: 100 tests
- Catalog number: KB03036-100T
- Manufacturer: Bioquochem
Ideal for plant biologists and agronomists studying stress tolerance mechanisms. Compatible with standard UV spectrophotometers. All reagents included with full protocol.
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Bioquochem je španielska biotech spoločnosť so sídlom v Astúrii, založená v roku 2015, ktorá vyvíja a vyrába rýchle spektrofotometrické súpravy a elektrochemické prístroje na kvantifikáciu redox parametrov – ako je celková antioxidačná kapacita, enzýmové aktivity, stres spojený s reaktívnymi kyslíkovými druhmi (ROS), obsah fenolických zlúčenín či poškodenie biomolekúl – v biologických, potravinárskych či kozmetických matriciach.